Bacillus anthracis

Ruling Out Bacillus anthracis

Optimization of methods for ruling out Bacillus anthracis leads to increased yields, faster turnaround times, and a lighter workload. We used 72 environmental non-B. anthracis bacilli to validate methods for ruling out B. anthracis. Most effective were the use of horse blood agar, motility testing after isolates had a 2-h incubation in trypticase soy broth, and screening isolates with a B. anth...

Lecithinase from Bacillus anthracis.

When Bacillus anthracis is grown in egg yolk medium, a reaction occurs which is presumably due to lecithinase (Colmer, 1948; McGaughey and Chu, 1948; Chu, 1949). In liquid egg yolk medium the lecithinase reaction is characterized by the formation of an opalescent suspension and flocculation of particles which rise to the top of the medium as a curd. A test such as this is usually referred to as...

Pyrosequencing Bacillus anthracis

Pyrosequencing technology is a sequencing method that screens DNA nucleotide incorporation in real time. A set of coupled enzymatic reactions, together with bioluminescence, detects incorporated nucleotides in the form of light pulses, which produces a profile of characteristic peaks in a pyrogram. We used this technology to identify the warfare agent Bacillus anthracis by sequencing 4 single n...

Transduction in Bacillus cereus and Bacillus anthracis.

A phage, designated CP-51, that carries out generalized transduction in Bacillus cereus and B. anthracis, has recently been isolated from soil. Transducing phages for members of the genus Bacillus have been described previously for B. subtilis and B. licheniformis (2-6). Those that have been best characterized are PBS-1 for B. subtilis (4) and SP-10 and SP-15 for both B. subtilis and B. licheni...

Bacillus anthracis isolated from aborted bovine fetuses